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Master Degree in
Advanced Molecular Sciences
Advanced and innovative analytical methods for applications in life sciences
Ilaria Palchetti
6 ECTS: 32 hours of lectures and 24 hours of laboratory training
Aims : The course deals with advanced analytical techniques and innovative methodologies for applications in the life sciences. The course deals with advanced analytical techniques and innovative methodologies for applications in the life sciences. We start from a description of the main analytical techniques used in the field of the omic sciences, bioanalysis, and bioelectrochemistry, try to understand the pros and cons of the different techniques described, and derive knowledge to optimally address the different issues encountered to solve different analytical problems in the life sciences.
In the process, we need to reflect on 1) what best/optimal/efficient methodology, and 2) what is the best outcome attainable given a particular analytical problem.
On completion of the course students will be able to identify and optimize sampling programs and experiments for answering questions in the field of life sciences; to identify the most appropriate analytical techniques; to formulate and interpret analytical chemistry data in the field of life sciences; to connect the outcome of the analytical chemistry interpretation and scientific progress in life sciences.
Lectures (4 ECTS):
- Analytical methodologies in the field of "omics" research (lectrophoresis in proteomics; Liquid Chromatography (HPLC, UPLC, 2D-LC, and nano LC) in metabolomics, lipidomics, proteomics; MS in proteomics; Analysis of Nucleic acids (Analytical PCR: quantitative PCR or real-time PCR, DNA microarrays, DNA sequencing);
- Affinity Assays (Immunoprecipitation and radioimmunoassay; Enzyme immunoassays (EIA and ELISA); Other Bioreceptors in Affinity assays; Lateral flow affinity-assays and POCT; Microbead-based affinity-assays and flow cytometry; Electrochemiluminescence affinity-assays)
- Bioelectrochemistry (The role of electrochemistry in life sciences and in vivo electroanalysis; Cell monitoring by using microelectrodes and nanoelectrodes; Imaging by using micro and nano electrodes”; Electroanalytical sensors and biosensors in clinical analysis; Impedance spectroscopy for cytotoxicity evaluation)
- Nanotechnology for bioanalysis
Lab practice (2 ECTS):
Assessment and Grading:
Midterm Assessment: Midterm written assesments on the topics of the course will be possible and decided with the students.
Exam: Oral test in order to assess the knowledge, the ability to apply knowledge, autonomy of judgment, the skills to discuss the issues related to analytical techniques.
Last
update
20.01.2024